Application of conjugate based on colloidal gold for express diagnostics of rabies in dot immunoanalysis

The aim of the research was to develop a direct dot-immunoassay on nitrocellulose membrane (NCM) using conjugate based colloidal gold for qualitative determination presence rabies virus antigen in pathological material. As test samples, we used brain samples various animal species, which were positive during initial study by methods fluorescent antibodies: foxes and mice. IFA indirect ELISA carried out diagnostic kits manufactured at Federal Center Toxicological, Radiation Biological Safety. materials present results primary laboratory tests system indication dotimmunoassay (DIA) 81 material, species. It shown that DIA has 100% specificity, its optical signals correlate with ELISA. proposed method, addition independent application, can also serve as basis design systems immunochemical analysis.